Development of EST-SSR markers of Ipomoea nil
نویسندگان
چکیده
Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives.
منابع مشابه
Characterization and development of EST-SSR markers in sweet potato (Ipomoea batatas (L.) Lam)
In this study, a cDNA library was constructed from the total RNA of sweet potato leaves. A total of 789 copies of the cDNA were cloned in Escherichia coli by employing the pGEM-T Easy vector. Sequencing was carried out by Solgent Co. (Korea). As many as 579 expressed sequence tag-simple sequence repeat (EST-SSR) markers were designed (73.38%) from the known cDNA nucleotide base sequences. The l...
متن کاملConstruction of a genetic map using EST-SSR markers and QTL analysis of major agronomic characters in hexaploid sweet potato (Ipomoea batatas (L.) Lam)
The Sweet potato, Ipomoea batatas (L.) Lam, is difficult to study in genetics and genomics because it is a hexaploid. The sweet potato study not have been performed domestically or internationally. In this study was performed to construct genetic map and quantitative trait loci (QTL) analysis. A total of 245 EST-SSR markers were developed, and the map was constructed by using 210 of those marke...
متن کاملGenetic Diversity and Population Structure of Iranian tulips revealed by EST-SSR and NBS-LRR Markers
The genus Tulipa L. (Liliaceae) comprises about 100 species and Iran is considered as one of the main origins of tulips. In this research, genetic diversity and population structure of 27 wild populations of tulips collected from Iran were studied by 15 highly polymorphic and reproducible expressed sequenced tag-simple sequence repeat (EST-SSR) markers and 8 nucleotide binding site (NBS)-enzyme...
متن کاملDetermination of Genetic diversity of cultivated chickpea (Cicer arietinum L.) using Medicago truncatula EST-SSRs
Expressed sequence tags simple sequence repeats (EST-SSRs) are important sources for investigation of genetic diversity and molecular marker development. Similar to genomic SSRs, the EST-SSRs are useful markers for many applications in genetics and plant breeding such as genetic diversity analysis, molecular mapping and cross-transferability across related species and genera. In spite of low po...
متن کاملبررسی تنوع ژنتیکی گیاه چمنشور ساحلی (Aeluropus littoralis) با استفاده از نشانگرهای ریزماهواره انتقالپذیر مبتنی بر EST
Aeluropus littoralis is one the most successful monocot grasses in terms of high salinity tolerance, that contains forage quality and has valuable physiological features. The goal of this study is to introduce and provide quick access to DNA-based molecular markers for valuable halophyte, Aeluropus littoralis to assessment of genetic diversity. For this, a total number of 110 EST-SSR ma...
متن کامل